The Renal Function Test Strip work with the Renal Function Analysis Meter（RFM-101）and Dry Biochemical Analysis Meter (DBM-101) to measure the Uric Acid(UA), Creatinine(CR/CREA) and Urea(UR/UREA) concentration in whole blood, plasma and serum. For professional use.

The 3-1 Renal Function Test Strip is used to measure the concentrations of Uric Acid (UA), Creatinine (CR) and Urea (UR). Renal Function measurements are used in the diagnosis various kidney diseases such as nephritis and renal insufficiency.

PRINCIPLE

The Renal Function Test Strip use a timed-endpoint method to measure the Uric Acid(UA),Creatinine(CR) and Urea (UR) concentrations in whole blood, serum or plasma. The system monitors the change in absorbance at 620 nm at a fixed-time interval. The change in absorbance is directly proportional to the concentration of metabolite in the specimen.

UA: In the reaction, the uric acid is oxidized to allantoin and hydrogen peroxide by uricase. Peroxidase catalyzes the reaction of hydrogen peroxide with 4-aminoantipyrine and phenol to produce a colored quinoneimine product. The change in absorbance is directly proportional to the concentration of uric acid in the specimen.

CR: Creatinine in the specimen are hydrolyzed to creatine by the action of creatininase. A sequence of three coupled enzymatic steps using creatinase, sarcosine oxidase, and peroxidase causes the oxidative coupling of 4-aminoantipyrine to form a blue dye.

UR: Urea in the specimen are hydrolyzed to NH3 and CO2 by the action of urease，and an indicator layer containing an indicator which produces a detectable change by gaseous ammonia.